组学

组学

       

背景 

针对组学实验,用户可以按特定需求,灵活应用标准操作步骤(预孵育,给样,冲洗等)和测试终点(生物化学,分子生物学,组学,细胞成像等),开发自己的测试方法和标准操作规范。与标准化的毒理学测试方法相比,该方法可以选择已知的参考物质作为阳性和阴性对照。

 

描述 

在三维体外重建组织模型出现的同时,组学技术经历了日新月异的快速发展。研究证明,三维组织模型比二维细胞培养更接近人体组织,并且与所有组学技术(基因组学,蛋白质组学,代谢组学等)兼容。应用体外组织模型可以比较对照组和样品组的差异,这是在体实验无法实现的,并可以更好地控制科学参数,以避免对实验结果产生可能的干扰。

 

应用实例: 

  • 比较单个或者多个供体 
  • 不同处理方法对实验结果的影响 

模型

EpiskinTM 表皮模型
SkinEthicTM RHE 表皮模型 

SkinEthicTM RHPE 含黑色素表皮模型 
T-Skin
T-SkinTM 全皮模型 

SkinEthicTM HCE 角膜模型   

 

详细检测步骤 

 

参考文献 

Adhesion of Staphylococcus aureus and Staphylococcus epidermidis to the EpiskinTM reconstructed epidermis model and to an inert 304 stainless steel substrate. G. Lerebour, S. Cupferman, M.N. Bellon-Fontaine. Journal of Applied Microbioloby, 97: 7-16, 2004


Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidosis. M. Schaller, H. C. Korting, W. Schäfer, J. Bastert, W. Chen, B. Hube. Molecular microbiology, 34: 169-180, 1999.


Effects of the Human Immunodeficiency Virus (HIV) proteinase inhibitors saquinavir and indinavir on in vitro activities of secreted aspartyl proteinases of Candida albicans isolates from HIV-infected patients. H. C. Korting, M. Schaller, G. Eder, G. Hamm, U. Böhmer, B. Hube. Antimicrobial Agents and Chemotherapy, 43: 2038-2042, 1999.


Toxicity and antimicrobial activity of a hydrocolloid dressing containing silver particles in an ex vivo model of cutaneous infection. M Schaller, J. Laude, H. Bodewaldt, G. Hamm, H. C. Korting. Skin Pharmacology and Physiology, 17: 31-36, 2004.


Polymorphonuclear leukocytes (PMNs) induce protective Th1-type cytokine epithelial responses in an in vitro model of oral candidiasis. M. Schaller, U. Boeld, S. Oberbauer, G. Hamm, B. Hube, H. C. Korting. Microbiology, 150: 2807-2813, 2004


An ultrastructural and a cytochemical study of candida invasion of reconstituted human oral epithelium. J. A. M. S. Jayatilake, Y. H. Samaranayake, L. P. Samaranayake. Journal of Oral pathology & Medicine, 34: 240-246, 2005.


RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium (RHE) model of oral candidiasis and in model biofilms. C. B. Green, G. Cheng, J. Chandra, P. Mukherjee. M. A. Ghannoum, L. Hoyer. Microbiology, 150: 267-275, 2004.

 

Hyphal invasion of Candida albicans inhibits the expression of human β-defensins in experimental oral candidiasis. Q. Lu, J. A. M. S. Jayatilake, L. P Samaranayake, L. Jin. Journal of investigative Dermatology, 126: 2049-2056, 2006.