细菌粘附

细菌粘附

   

背景

针对细菌粘附实验的测试方法,用户可以按特定需求,灵活应用标准操作步骤(预孵育,给样,冲洗等)和测试终点(生物化学,分子生物学,组学,细胞成像等),开发自己的测试方法和标准操作规范。与标准化的毒理学测试方法相比,该方法可以选择已知的参考物质作为阳性和阴性对照。

 

描述

近期的科学研究证实细菌对人类上皮和表皮有重要的影响,因为三维重建组织模型可以被共生菌或病原体细菌粘附,所以此类模型可以作为研究上皮/皮肤微生物的工具。(请注意订购没有抗生素的特定培养基)

应用实例

  • 研究细菌粘附机理
  • 研究和治疗念珠菌病

 

模型

EpiSkinTM 表皮模型(大孔径)

SkinEthicTM HCE 角膜模型
 
 
 
 
 
 
 
 

 

详细实验步骤

 

参考文献

Adhesion of Staphylococcus aureus and Staphylococcus epidermidis to the EpiSkinTM reconstructed epidermis model and to an inert 304 stainless steel substrate. G. Lerebour, S. Cupferman, M.N. Bellon-Fontaine. Journal of Applied Microbioloby, 97: 7-16, 2004


Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidosis. M. Schaller, H. C. Korting, W. Schäfer, J. Bastert, W. Chen, B. Hube. Molecular microbiology, 34: 169-180, 1999.


Effects of the Human Immunodeficiency Virus (HIV) proteinase inhibitors saquinavir and indinavir on in vitro activities of secreted aspartyl proteinases of Candida albicans isolates from HIV-infected patients. H. C. Korting, M. Schaller, G. Eder, G. Hamm, U. Böhmer, B. Hube. Antimicrobial Agents and Chemotherapy, 43: 2038-2042, 1999.


Toxicity and antimicrobial activity of a hydrocolloid dressing containing silver particles in an ex vivo model of cutaneous infection. M Schaller, J. Laude, H. Bodewaldt, G. Hamm, H. C. Korting. Skin Pharmacology and Physiology, 17: 31-36, 2004.


Polymorphonuclear leukocytes (PMNs) induce protective Th1-type cytokine epithelial responses in an in vitro model of oral candidiasis. M. Schaller, U. Boeld, S. Oberbauer, G. Hamm, B. Hube, H. C. Korting. Microbiology, 150: 2807-2813, 2004


An ultrastructural and a cytochemical study of candida invasion of reconstituted human oral epithelium. J. A. M. S. Jayatilake, Y. H. Samaranayake, L. P. Samaranayake. Journal of Oral pathology & Medicine, 34: 240-246, 2005.


RT-PCR detection of Candida albicans ALS gene expression in the reconstituted human epithelium (RHE) model of oral candidiasis and in model biofilms. C. B. Green, G. Cheng, J. Chandra, P. Mukherjee. M. A. Ghannoum, L. Hoyer. Microbiology, 150: 267-275, 2004.

 

Hyphal invasion of Candida albicans inhibits the expression of human β-defensins in experimental oral candidiasis. Q. Lu, J. A. M. S. Jayatilake, L. P Samaranayake, L. Jin. Journal of investigative Dermatology, 126: 2049-2056, 2006.